The spatial organization of the cellular cytoplasm has fascinated cell biologists, particularly since the introduction of advanced microscopy techniques. We are interested in elucidating how complex microtubule arrays, on the micron scale, are organized and their functional roles in critical cellular processes, such as mitotic progression, ciliogenesis, and neuronal maturation. Moreover, we examine how nuclear transport factors modulate the organization of microtubule-based structures (e.g., spindle and anemone) via canonical and non-canonical activities. Beyond fundamental cell biology, my research extends to understanding the molecular pathways underlying cancer drug resistance. Given the significance of tubulin as a target for anti-cancer drugs, we are focusing on elucidating the mechanisms responsible for drug resistance. This study aims to deepen our understanding of cancer drug resistance and potentially uncover new avenues for the development of more effective cancer treatments.

 

Our methodology involves the reconstitution and imaging of microtubule-based structures from their constituent protein subunits. This is complemented by an interdisciplinary approach, incorporating techniques from structural biology, biophysics, and cellular biology to uncover regulatory mechanisms. Through this integrative approach, we aim to discover the mechanistic links between the cytoskeletal organization and vital cell functions. Ultimately, our goal is to reveal the mechanistic connections between cytoskeletal organization and essential cellular functions, with potential implications for both basic science and therapeutic applications.